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Whole Genome Assembly of M. acuminata DH Pahang (v2)

Genotyping by sequencing (GBS) of a segregating population and paired-end sequencing were used to detect and correct scaffold misassemblies. Long insert size paired-end reads identified scaffold junctions and fusions missed by automated assembly methods. GBS markers were used to anchor scaffolds to pseudo-molecules with a new bioinformatics approach that avoids the tedious step of marker ordering during genetic map construction. Furthermore, a genome map was constructed and used to assemble scaffolds into super scaffolds.

Program, Pipeline, Workflow or Method Name
8 successive steps based on Scaffremodler and Scaffhunter
Program Version
Date Performed
Monday, October 28, 2019 - 17:48
Data Source
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Cross Reference
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Martin G, Baurens FC, Droc G, Rouard M, Cenci A, Kilian A, Hastie A, Dole┼żel J, Aury JM, Alberti A, Carreel F, D'Hont A. Improvement of the banana "Musa acuminata" reference sequence using NGS data and semi-automated bioinformatics methods.. BMC genomics. 2016 Mar 16; 17:243.